There are four major topics for the course:
1. Introduction and laboratory requirements for cell culture
2. Methods for cell growth and propagation of continuous cell lines
3. Methods for isolation and culture of primary cell lines
4. Pharmaceutical applications of cell culture: CaCO-2 and OK cells

1. Introduction and laboratory requirements for cell culture
(Each class represents approximately 1 hour of lecture time).

Classes 1 & 2: Tissue Culture in Biomedical Research; Growth of Cultured Cells: History of an Established Cell Line
Class 3: Physical and Chemical Requirements for Cell Culture; Lab Safety and Proper Use of Biohazard Hoods

Mini-Laboratory Exercise 1.
Subculturing of Cells: Sterile Technique, Cell Enumeration and Cell Viability;
Examination of Growth Patterns of Various Types of Cells

Classes 4 & 5: Nutrition and Growth Control: Media Formulations, Serum, Growth Factors,
Serum-Free Media
Class 6: Media Preparation and Sterilization
Class 7: Cell Transformation and Properties of Transformed Cells; Low Density and Clonal Growth, Cyropreservation
Class 8: Sterility: Testing Regimens and Quality Control; Mycoplasma Contamination Cell Line Identification

Mini- Laboratory Exercise 2.
Low Density and Clonal Growth; Cloning of Cells; Cyropreservation
Culture Techniques for Growing a Cell Line from a Frozen Stock

Class 9: Considerations in the Sterile Harvesting of Tissue; Tissue Dissociation Methods
Class 10: Special Needs of Primary Cell Cultures: Substrata, Nutrient Supplements, Growth Factor Supplements; Co-cultures

Mini-Laboratory Exercise 3
Chick Embryo Primary Cell Cultures

Class 11, 12 & 13: Culture of CaCO-2 and OK cells; CaCO-2 cells as a Model of Intestinal Drug
Absorption; OK cells as a Model of Kidney Active Transport Processes

Mini-Laboratory Exercise 4:
Subculturing of CaCO-2 Cells; Active Uptake of Amino Acids by CaCO-2 Cells

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