Molecular Genetic Methodologies for Pharmaceutical Sciences
(PHC 543)
January 8 - 12, 2001
Daily Schedule
Monday, 8 January
|
9:00 |
- | 10:00 | Introduction |
| 10:00 | - | 11:30 | Lab: Isolation of total RNA |
| 11:30 | - | 12:30 | Lunch |
| 12:30 | - | 1:30 | Lab: Gel electrophoresis of total RNA |
| 1:30 | - | 2:30 | Lecture: Primer on nucleic acids and genomes |
| 2:30 | - | 3:00 | Break |
| 3:00 | - | 4:00 | Lecture: Primer on genetics |
| 4:00 | - | 5:00 | Discussion: Lab results |
Tuesday, 9 January
|
9:00 |
- | 10:00 | Lab: cDNA synthesis |
| 10:00 | - | 11:00 | Lecture: Enzymes in molecular biology |
| 11:00 | - | 12:00 | Lecture: Polymerase chain reaction I |
| 12:00 | - | 1:00 | Lunch |
| 1:00 | - | 2:30 | Lab: RT-PCR |
| 2:30 | - | 3:30 | Lecture: Polymerase chain reaction II |
| 3:30 | - | 4:00 | Break |
| 4:00 | - | 5:00 | Lecture: Bioinformatics & Molecular Biology Databases |
|
9:00 |
- | 9:30 | Lab: Gel fractionation of PCR products |
| 9:30 | - | 10:30 | Lecture: Nucleic acid hybridization assays |
| 10:30 | - | 11:30 | Lab: RNA probe synthesis |
| 11:30 | - | 12:00 | Lab: Analysis of PCR fragments |
| 12:00 | - | 1:00 | Lunch |
| 1:00 | - | 2:00 | Lab: Pre-hybridization |
| 2:00 | - | 3:00 | Lab: Cloning of PCR products - ligation |
| 3:00 | - | 3:30 | Break |
| 3:30 | - | 4:00 | Lab: Hybridization |
| 4:00 | - | 5:00 | Lab: Cloning of PCR products - transformation |
|
9:00 |
- | 11:00 | Lab: Chemiluminescent detection |
| 11:00 | - | 12:00 | Lecture: Ligation reactions |
| 12:00 | - | 1:00 | Lunch |
| 1:00 | - | 2:30 | Lecture: Array analysis |
| 2:30 | - | 3:00 | Break |
| 3:00 | - | 4:30 | Lecture: Cloning strategies |
| 4:30 | - | 5:00 | Lab: Positive colony selection and setup of overnight cultures |
|
9:00 |
- | 10:00 | Lab: Mini-preparation of plasmid DNA |
| 10:00 | - | 11:00 | Lab: Restriction digestion and gel fractionation |
| 11:00 | - | 12:00 | Lab: Analysis of restriction digestions |
| 12:00 | - | 1:00 | Lunch |
| 1:00 | - | 3:00 | Discussion: Lab results |
| 3:00 | - | 3:15 | Workshop Conclusion and Evaluation |