DNA/cDNA Arrays

The Pharmaceutical Genetics Lab has a number of DNA "mini"-arrays available to faculty who would like to try DNA microarray technology (for example as preliminary data for grant applications).  The PGL can provide the necessary reagents and training for faculty who would like to explore this new technology.  The "mini-arrays" we have are GEArray^TM pathway-specific expression arrays kits from Super Array.   Each kits contains two identical cDNA array membranes (for treatment and control comparisons) as well as most of the reagents necessary for hybridization experiments.

For more information concerning the actual genes present on the membranes please visit Super Array Inc. website.  The descriptions below come the company's website.  

GEArray^TM pathway-specific expression arrays are efficient and easily used research tools for determining pathway-specific gene expression profiling. Each pathway-specific array is a positively charged nylon membrane on which more than 20 cDNA fragments from genes associated with a particular signal transduction pathway have been immobilized in duplicate. Two identical membranes spotted with pathway-specific cDNA fragments are included in each kit. The expression profiles of two mRNA populations can be compared to determine specific genes that are regulated in a particular pathway under differential conditions.

The Human GEArray Kits are designed to help characterize the gene expression profiles associated with experimental treatments, disease conditions and developmental stimuli. Through a simple side-by-side hybridization with your experimental and control RNA samples using GEArrays and the reagents provided, you can determine simultaneously whether these apoptosis-related genes are induced or suppressed in response to your experimental conditions.

The Mouse GEArray Kits are designed to help characterize the gene expression profiles associated with experimental treatments, disease conditions and developmental stimuli. Through a simple side-by-side hybridization with your experimental and control RNA samples using GEArrays and the reagents provided, you can determine simultaneously whether these cell cycle-related genes are induced or suppressed in response to your experimental conditions. These genes include various members of cyclins, cyclin-dependent kinases (CDKs), CDK inhibitors, and other key genes that affect the entry, withdrawal and progression of the cell cycle.

The GEARRay kits available in the PGL include:

Human GEArray kits:

• Human Cellcycle-2 GEArray Kit
• Human Cancer/Angiogenesis-1 GEArray Kit

Mouse GEArray kits (can be used in rat studies):

• Mouse Apoptosis-1 GEArray Kit
• Mouse Apoptosis-2 GEArray Kit
• Mouse Cancer/Angiogenesis-2 GEArray Kit
• Mouse PathwayFinder-1 GEArray Kit
• Mouse Common Cytokin GEArray Kit
• Mouse Chemokines GEArray Kit
• Mouse NFkB Pathway GEArray Kit

Please contact the PGL if you would like to try this new technology.

Dr. Daniel A. Brazeau
Department of Pharmaceutical Sciences
(716) 645-2842 Ext. 260

Last modified 18 December 2000